Curcumin HPLC

High performance liquid chromotography, or HPLC, is a technique used in chemistry to separate, identify, and quantify each component in a mixture. The pumps switch between solvents A and B. Solvent A was composed of 2% acetic acid and 98% water, while solvent B was composed of 2% acetic acid and 98% acetonitrile. The LC Time Progress was set for 50% of solvent B at 1:00 minute, 53% of solvent B at 3:00 minutes, and 56% of solvent B at 5:00 minutes; at fifteen minutes the run was stopped. 

First, an HPLC was run for a 6.78 x 10^-3M sample of Curcumin. 

Second, an HPLC was run for a sample of curcumin one hundred times more concentrated than the first run.

Next, an HPLC was run for a 1:5-Iron:Curcumin ratio at 420nm.

Finally, an HPLC was run for a 5:1-Iron:Curcumin ratio at 420nm.

5/30/2018
-Kaitlyn Jordan