TLC- Thin Layer Chromatography

Once the solution was purified, a TLC was run to see if:
1. The sample was pure.
2. Three distinct spots were visible, meaning all three curcuminoids were present in solution.

The solvent used in the TLC chamber was a 95:5 mixture of CH₂Cl₂:CH₃OH.
Two spots of the filtered curcumin sample were used initially.
The resulting plate looked as follows:

The result produced three distinct spots for each original run.  This means that all three curcuminoids were in solution and the solvent in the chamber was appropriate for the test.

The spots were assigned designations A, B, and C, from top to bottom, meaning that the spot that traveled 4.10cm was A.

The Rf values were:
Rf A- 0.788
Rf B- 0.654
Rf C- 0.558





-Alex Griffith